Library prep
Read balancing is the process of adjusting library ratios so every sample receives a comparable share of the sequencing output.
Without it, abundant samples dominate the run and scarce ones drop below usable depth, wasting capacity that was already paid for.
Quantification is the process of measuring the concentration of DNA in a sample, typically before pooling and sequencing.
Pooling is the process of combining multiple indexed libraries into one tube so they can be sequenced together in a single run.
Normalization is the process of equalizing the concentration of different DNA libraries so each is represented evenly when pooled.
Library preparation is the multi-step process of converting DNA or RNA samples into a collection of sequencing-ready fragments.
Library clean up is the step that removes leftover reagents and unwanted reaction byproducts from a library before sequencing.
.svg){kind=icon}
