Library prep
Library clean up is the step that removes leftover reagents and unwanted reaction byproducts from a library before sequencing.
Clean up removes short artifacts such as adapter dimers, but it cannot recover a library that was already over-amplified.
Read balancing is the process of adjusting library ratios so every sample receives a comparable share of the sequencing output.
Quantification is the process of measuring the concentration of DNA in a sample, typically before pooling and sequencing.
Pooling is the process of combining multiple indexed libraries into one tube so they can be sequenced together in a single run.
Normalization is the process of equalizing the concentration of different DNA libraries so each is represented evenly when pooled.
Library preparation is the multi-step process of converting DNA or RNA samples into a collection of sequencing-ready fragments.
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