Library prep
Pooling is the process of combining multiple indexed libraries into one tube so they can be sequenced together in a single run.
Pooling only works well if the libraries were normalized first, otherwise the most concentrated sample takes a disproportionate share of the reads.
Read balancing is the process of adjusting library ratios so every sample receives a comparable share of the sequencing output.
Quantification is the process of measuring the concentration of DNA in a sample, typically before pooling and sequencing.
Normalization is the process of equalizing the concentration of different DNA libraries so each is represented evenly when pooled.
Library preparation is the multi-step process of converting DNA or RNA samples into a collection of sequencing-ready fragments.
Library clean up is the step that removes leftover reagents and unwanted reaction byproducts from a library before sequencing.
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