Library prep
Library preparation is the multi-step process of converting DNA or RNA samples into a collection of sequencing-ready fragments.
A typical workflow runs fragmentation, adapter ligation, amplification, clean up, quantification, normalization, and pooling.
Read balancing is the process of adjusting library ratios so every sample receives a comparable share of the sequencing output.
Quantification is the process of measuring the concentration of DNA in a sample, typically before pooling and sequencing.
Pooling is the process of combining multiple indexed libraries into one tube so they can be sequenced together in a single run.
Normalization is the process of equalizing the concentration of different DNA libraries so each is represented evenly when pooled.
Library clean up is the step that removes leftover reagents and unwanted reaction byproducts from a library before sequencing.
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