All glossary terms

Library prep

Amplification

Amplification is the process of creating many copies of a targeted DNA sequence, usually by PCR.

What is amplification in library prep?

It is the step that rescues low-input samples and also the step that generates most artifacts, which is why when it stops matters as much as that it happens.

Read Balancing

Read balancing is the process of adjusting library ratios so every sample receives a comparable share of the sequencing output.

Quantification

Quantification is the process of measuring the concentration of DNA in a sample, typically before pooling and sequencing.

Pooling

Pooling is the process of combining multiple indexed libraries into one tube so they can be sequenced together in a single run.

Normalization

Normalization is the process of equalizing the concentration of different DNA libraries so each is represented evenly when pooled.

Library Preparation

Library preparation is the multi-step process of converting DNA or RNA samples into a collection of sequencing-ready fragments.

Library Clean Up

Library clean up is the step that removes leftover reagents and unwanted reaction byproducts from a library before sequencing.