NGS basics
The RNA Integrity Number (RIN) is a numerical score, from 1 to 10, used to rate the quality of an RNA sample.
RNA degrades faster than DNA, so RIN is often the first thing checked before committing a sample to library prep.
Sequencing is the process of reading the nucleotide order (A, T, C, G) of DNA or RNA fragments.
Read depth is the total number of individual sequence reads generated for a given sample or region.
Primers are short single-stranded sequences that bind a DNA template and act as the starting point for DNA synthesis.
The polymerase chain reaction (PCR) is a technique that copies a specific DNA sequence exponentially through repeated temperature cycles.
Next generation sequencing (NGS) is a high-throughput technology that reads millions of DNA or RNA fragments in parallel.
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