NGS basics
Primers are short single-stranded sequences that bind a DNA template and act as the starting point for DNA synthesis.
Primers that bind each other instead of the template form primer dimers, one of the most common sources of baseline noise in real-time detection.
Sequencing is the process of reading the nucleotide order (A, T, C, G) of DNA or RNA fragments.
The RNA Integrity Number (RIN) is a numerical score, from 1 to 10, used to rate the quality of an RNA sample.
Read depth is the total number of individual sequence reads generated for a given sample or region.
The polymerase chain reaction (PCR) is a technique that copies a specific DNA sequence exponentially through repeated temperature cycles.
Next generation sequencing (NGS) is a high-throughput technology that reads millions of DNA or RNA fragments in parallel.
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