NGS basics
Low-input and degraded samples are specimens with scarce or damaged starting material, such as cfDNA from plasma or DNA from FFPE tissue.
These samples are usually irreplaceable and have the least tolerance for a failed run, which is exactly where fixed-cycle amplification is riskiest.
Sequencing is the process of reading the nucleotide order (A, T, C, G) of DNA or RNA fragments.
The RNA Integrity Number (RIN) is a numerical score, from 1 to 10, used to rate the quality of an RNA sample.
Read depth is the total number of individual sequence reads generated for a given sample or region.
Primers are short single-stranded sequences that bind a DNA template and act as the starting point for DNA synthesis.
The polymerase chain reaction (PCR) is a technique that copies a specific DNA sequence exponentially through repeated temperature cycles.
Next generation sequencing (NGS) is a high-throughput technology that reads millions of DNA or RNA fragments in parallel.
.svg){kind=icon}
