NGS basics
Amplicons are the copied DNA or RNA fragments produced by an amplification reaction such as PCR.
The word describes the product, not the process: a clean library and a plate full of artifacts are both made of amplicons.
Sequencing is the process of reading the nucleotide order (A, T, C, G) of DNA or RNA fragments.
The RNA Integrity Number (RIN) is a numerical score, from 1 to 10, used to rate the quality of an RNA sample.
Read depth is the total number of individual sequence reads generated for a given sample or region.
Primers are short single-stranded sequences that bind a DNA template and act as the starting point for DNA synthesis.
The polymerase chain reaction (PCR) is a technique that copies a specific DNA sequence exponentially through repeated temperature cycles.
Next generation sequencing (NGS) is a high-throughput technology that reads millions of DNA or RNA fragments in parallel.
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