PCR artifacts
Primer dimers are artifacts formed when two primer molecules hybridize to each other instead of to the target DNA template.
They fluoresce like real product at standard scan temperatures, which is why HotScan reads above their melting point to keep them out of the signal.
Size bias is the tendency of PCR to preferentially amplify shorter fragments, distorting the original fragment size distribution.
Recombination is the unintended shuffling of genetic material between fragments, often driven by over-amplification.
Jackpotting is the runaway over-amplification of a few random fragments, which skews their representation in the sequencing data.
Chimeras are artifacts formed when fragments from different DNA molecules fuse into a single artificial hybrid sequence.
Adapter dimers are artifacts formed when two sequencing adapters ligate to each other instead of to a DNA insert.
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