UnlockingFaster, Smarter PCR Workflows for 2025 and Beyond

Assay development is at the core of scientificdiscovery in genomics, diagnostics, and life science. Yet, optimizing PCRworkflows has always meant battling with split-plot constraints, endlessthermocycler runs, and mountains of consumable waste. If you’re seeking agame-changer for your lab, it's time to meet iconPCR with AutoNorm™—thebenchtop thermocycler reimagined by n6 for the next-generation scientist.

Why PCR Assay Development Needs a FreshApproach

PCR assay development relies on fine-tuningmultiple factors: enzyme concentrations, buffer chemistry, oligonucleotidelevels, and—critically—thermocycler conditions. Traditional thermocyclers forceyou to compromise. All wells on a plate share the same conditions, meaningcomplex experiments split across plates, slow project timelines, increasedreagent costs, and missed optimization opportunities. This becomes especiallypainful during Design of Experiments (DOE) studies, where exploring multiplevariables is essential.

How iconPCR Makes DOE in PCR AssayDevelopment Effortless

With iconPCR, every well operates as its ownindependent reactor. The platform unlocks:

  • 96 fully independent wells—run different temperatures, cycle counts,and conditions in a single experiment.
  • More than 80°C of linear gradients—expand your parameter search withoutextra plates.
  • Built-in AutoNorm real-time normalization—achieve consistent, reliableamplification and hassle-free pooling.
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This means you can optimize across multiplefactors in a single run—no more split-plot designs, no more wasted plates. Forexample, a classic DOE with six reagent parameters and multiple thermocyclersettings (as modeled after the Whitcomb & Kraber design) could require 15to 60 plates on legacy systems, soaking up 20 to 100 hours of instrument time.iconPCR slashes that down to as few as 5–20 plates, delivering up to 80% plateand time savings.​

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Data-DrivenScalability for Every Lab

iconPCR isn’t just fast. It’s smart, scalable,and designed for real-world workflows:

  • Add experimental factors on the fly. Want to explore one moretemperature, time, or condition? Simply assign it to available wells—no needfor extra plates.
  • Eliminate edge effects. Each well is independently monitored andheated, so you leverage the full capacity of your plate.
  • Richer data, fewer headaches. Run deeper, more informative DOE screensin a fraction of the time.

Make Every Experiment Count—and Save

By bringing full-factorial, combinatorial powerto a tabletop instrument, iconPCR ensures you get more data with less waste.The more complex your optimization, the bigger the savings: fewer plates,dramatically lower reagent costs, and an eco-friendly reduction in plasticwaste.

Ready to Revolutionize Your Workflow?

Every PCR assay development project isunique—but they all deserve efficiency. Discover how iconPCR can fast-trackyour optimization, reduce consumable usage, and make high-quality DOE studieseasy.

Are you ready to streamline your next bigproject? Contactn6 today or visit our website to see iconPCR in action and downloadthe full application note.

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